SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Cuyeu,Romina; Rosso,Beatriz; Pagano,Elba; Soto,Gabriela; Fox,Romina; Ayub,Nicolás Daniel.

Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of...

Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)

Palavras-chave: SSR markers; Genetic diversity; Flow cytometry; Tall fescue; Genetic resources.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200014

Contim,Luis Antônio Serrão; Carvalho,Carlos Roberto de; Martins,Franciele Alline; Freitas,Danival Vieira de.

Rosewood (Aniba rosaeodora Ducke, Lauraceae) is ecologically and economically important to the Amazon region. As a consequence of its economic importance, rosewood populations have been decimated in the Amazon forest. Species of nine genera of the Lauraceae family have characterized karyotypes with n = x = 12 chromosomes in the gametophytic phase but the genus Aniba is one of the least studied Lauraceae genera with a previously undescribed genome. We used cytogenetic techniques to determine that the A. rosaeodora karyotype contained 12 pairs (2n = 24) of relatively small submetacentric chromosomes with lengths ranging from 1.34 to 2.25 mum and a nucleolar organizer region (NOR) in the short arm of chromosome 7. Flow cytometry gave 2C = 2.32 pg of DNA,...

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)

Palavras-chave: Rosewood; Aniba rosaeodora; Lauraceae; Karyotype; Flow cytometry.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500017

Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.

When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.

Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa

Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019

Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J..

The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...

Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum

Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016

Monteiro,Rita; Carvalho,Cláudia; Collares-Pereira,Maria João.

This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both...

Spatial and temporal variability of the pico- and nanophytoplankton and bacterioplankton in a deep Polynesian atoll lagoon

Palavras-chave: Ag/CMA3-banding; Flow cytometry; NORs; Chromosome polymorphism; Spontaneous triploidy.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200011

Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas

Thomas, Y.; Garen, Pierre; Courties, C.; Charpy, L..

Spatial and temporal scales of variability in phyto- and bacterioplankton abundance were investigated in a deep atoll lagoon (Ahe, French Polynesia), Ahe Lagoon showed a classical phytoplankton composition, but its picophytoplankton (Prochlorococcus, Synechococcus and picoeukaryotes) concentrations are higher than the mean for Polynesian atoll lagoons. Phyto- and bacterioplankton descriptors were highly variable, with mean CVs of >20% in most cases: day night, day-to-day, vertical, lagoonal and seasonal. Phytoplankton biomass, which was estimated by in vivo fluorescence, was higher in the deep layer than in the upper layer. This pattern was detected on a day night scale, showing transitory vertical gradients, and through long-term events that were...

Tipo: Text

Palavras-chave: Flow cytometry; Picophytoplankton; Nanophytoplankton; Bacterioplankton; Vertical distribution; Day-to-day dynamics; Large-scale heterogeneity; Deep atoll lagoon; French Polynesia.

Ano: 2010

URL: http://archimer.ifremer.fr/doc/00003/11425/7963.pdf

Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis.

Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced...

Tipo: Text

Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity.

Ano: 2006

URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Stachowski-haberkorn, Sabine; Quiniou, Louis; Beker, Beatriz; Haberkorn, Hansy; Marie, Dominique; De La Broise, Denis.

The impacts of the fungicide Opus(A (R)) (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 mu g l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 mu g l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant...

Tipo: Text

Palavras-chave: Microcosm; Phytoplankton community; Epoxiconazole; TTGE; Flow cytometry; HPLC.

Ano: 2009

URL: http://archimer.ifremer.fr/doc/00088/19888/17547.pdf

Seasonal to hour variation scales in abundance and production of total and particle-attached bacteria in the open NW Mediterranean Sea (0-1000 m)

Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes: Screening of 23 molecules using flow cytometry

Mevel, G.; Vernet, M.; Goutx, M.; Ghiglione, J. F..

We present the vertical and temporal dynamics of total vs. particle-attached bacterial abundance and activity over a 5 week period under summer to autumn transition in NW Mediterranean Sea. At a weekly time scale, total bacterial biomass and production in the euphotic layers was significantly correlated with phytoplanktonic biomass. At an hourly time scale, total bacterial biomass responded very rapidly to chlorophyll a fluctuations, suggesting a tight coupling between phytoplankton and bacteria for resource partitioning during the summer-autumn transition. In contrast, no influence of diel changes on bacterial parameters was detected. Episodic events such as coastal water intrusions had a significant positive effect on total bacterial abundance and...

Tipo: Text

Palavras-chave: Nucleic acid content; Free living bacteria; Top down control; Flow cytometry; Atlantic ocean; Water column; Spring bloom; Bottom up; North sea; Biogeochemical signifiance.

Ano: 2008

URL: https://archimer.ifremer.fr/doc/00208/31973/30408.pdf

Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan.

The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed...

Tipo: Text

Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities.

Ano: 2006

URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf

A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas

Short-term interactions between phytoplankton and intertidal seagrass vegetation in a coastal lagoon (Bassin d'Arcachon, SW France)

Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.

Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...

Tipo: Text

Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.

Ano: 2019

URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

De Wit, Rutger; Troussellier, Marc; Courties, Claude; Buffan-dubau, Evelyne; Lemaire, Emmanuelle.

We experimentally simulated the temporality of the interactions between intertidal seagrasses and phytoplankton in the context of nutrient enrichment from continental origin. Phytoplankton development was retarded after 1-day exposure to intertidal Zostera noltii Hornemann vegetation samples, with respect to control treatments. This was not explained by resource competition and we hypothesized a direct interference between seagrass leaves and phytoplankton. After separation from the vegetation in 5-day incubations, the final yield of large phytoplankton, mainly diatoms and haptophytes, was determined by nutrient loading. In contrast, Synechococcus-like cells (SYN), phototrophic picoeukaryotes and nanophytoplankton decreased after day 3, most likely due to...

Tipo: Text

Palavras-chave: Competition; Allelopathy; Zostera noltii; Sediment; Intertidal vegetation; Flow cytometry; Photosynthetic pigments.

Ano: 2012

URL: http://archimer.ifremer.fr/doc/00106/21717/19414.pdf

Diploid and triploid Pacific oysters, Crassostrea gigas (Thunberg), reared at two heights above sediment in Marennes-Oleron Basin, France: Difference in mortality, sexual maturation and hemocyte parameters

Gagnaire, Beatrice; Soletchnik, Patrick; Madec, Patrick; Geairon, Philippe; Le Moine, Olivier; Renault, Tristan.

Summer mortality is an important economic concern for the Pacific oyster, Crassostrea gigas, industry all over the world and particularly in France. Outbreaks appear when oysters are in gonadal maturation. Bivalve defence mechanisms against pathogen invasion are assumed by circulating cells, the hemocytes. A field experiment was conducted in the Marennes-Oleron Basin (Charente-Maritime, France) in order to monitor hemocyte parameters, gametogenesis and mortality rates among C. gigas during 7 months from March to September 2002. Diploid (from natural bed and from hatchery) and triploid oysters were placed at 15 cm or 70 cm above sediment at Marennes-Oleron Basin. Triploid animals were included because they are considered as sterile animals. Mortality rates...

Tipo: Text

Palavras-chave: Sexual maturation; Summer mortality; Triploids; Diploids; Flow cytometry; Hemocyte activities; Crassostrea gigas; Pacific oyster.

Ano: 2006

URL: http://archimer.ifremer.fr/doc/2006/publication-2923.pdf

Spatial and temporal extension of eutrophication associated with shrimp farm wastewater discharges in the New Caledonia lagoon

Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites

Thomas, Yoann; Courties, Claude; El Helwe, Yasmin; Herbland, Alain; Lemonnier, Hugues.

Shrimp farming in New Caledonia typically uses a flow-through system with water exchange rates as a tool to maintain optimum hydrological and biological parameters for the crop. Moreover, the effluent shows hydrobiological characteristics (minerals, phytoplankton biomass and organic matter) significantly higher than that of the receiving environment. Separate surveys were carried out in a bay (CH Bay) with a medium-size intensive farm (30 ha) (PO) and in a mangrove-lined creek (TE Creek) near a larger semi-intensive farm (133 ha) (SO). Net loads of nitrogen exported from the semi-intensive farm and the intensive farm amounted to 0.68 and 1.36 kg ha(-1) day(-1), respectively. At CH Bay, discharge effects were spatially limited and clearly restricted to...

Tipo: Text

Palavras-chave: Environmental impact; Shrimp farming; Eutrophication; Flow cytometry; New Caledonia.

Ano: 2010

URL: http://archimer.ifremer.fr/doc/00014/12527/9882.pdf